The Steps of Genetic Engineering

At IGCSE level, you need to know the basic steps:

  1. Identify the gene for the desired characteristic in the donor organism
  2. Cut the gene from the donor DNA using restriction enzymes (biological scissors)
  3. Insert the gene into a vector (usually a bacterial plasmid) using ligase enzyme
  4. Transfer the vector into the host organism (e.g. A bacterium)
  5. The host organism now produces the desired protein coded by the inserted gene

The most important example is the production of human insulin by bacteria. The human insulin gene is inserted into E. Coli bacteria, which then produce human insulin in large quantities through fermentation.

Advantages and Concerns

Advantages of genetic engineering over selective breeding:

  • Much faster: no need to wait for many generations
  • More precise: only the desired gene is transferred
  • Can cross species barriers: genes from any organism can be used
  • Can produce substances that selective breeding cannot (e.g. Human proteins in bacteria)

Concerns about genetic engineering:

  • GM organisms could escape and affect wild populations
  • Inserted genes could have unexpected effects
  • Ethical concerns about modifying living organisms
  • Long-term health effects of GM foods are debated
  • Could be used irresponsibly (e.g. Biological weapons, designer babies)