Anaerobic respiration in yeast

Yeast is a single-celled fungus that can respire both aerobically and anaerobically. In the absence of oxygen, yeast carries out anaerobic respiration (also called fermentation):

glucose → ethanol + carbon dioxide

C6H12O6 → 2C2H5OH + 2CO2

This process releases a small amount of energy compared to aerobic respiration because the glucose is only partially broken down. The ethanol still contains chemical energy.

Bread making

In bread making, yeast is mixed with flour (which contains starch), water and sugar. The yeast produces enzymes that break down some starch into glucose. The yeast then ferments the glucose, producing carbon dioxide and ethanol.

The carbon dioxide gas forms bubbles in the dough, causing it to rise and giving bread its light, spongy texture. The dough is then baked at a high temperature, which kills the yeast, evaporates the ethanol, and sets the structure. The small amount of ethanol produced evaporates during baking.

Beer making

In beer production, barley grains are allowed to germinate (malting), which activates enzymes that break down starch into maltose (a sugar). The germination is then stopped by heating. The maltose is dissolved in water and hops are added for flavour.

Yeast is added to the liquid, and fermentation occurs in sealed vessels. The yeast converts the sugars into ethanol (the alcohol in beer) and carbon dioxide (which provides the fizz or carbonation). The conditions are kept anaerobic by sealing the vessel, and at a suitable temperature (around 25-30 degrees C) for optimum yeast enzyme activity.

Conditions for fermentation

Successful fermentation requires: a sugar substrate (glucose or maltose), yeast as the microorganism, anaerobic conditions (absence of oxygen), and a suitable temperature (warm but not too hot, typically 25-35 degrees C).

If the temperature is too low, yeast enzymes work slowly and fermentation is slow. If it is too high, the yeast enzymes denature and fermentation stops. If oxygen is present, yeast respires aerobically, producing carbon dioxide and water instead of ethanol.