Priority 1: Food chains and webs

Arrows show direction of energy transfer (prey → predator). Trophic levels: producer → primary consumer → secondary consumer → tertiary consumer. Be able to predict effects of removing one organism from a food web.

Priority 2: Energy transfer

Energy is lost at each trophic level through: respiration (heat), egestion (undigested material), and excretion. This is why food chains rarely have more than 4–5 trophic levels. Pyramid of energy always has a pyramid shape (unlike pyramids of numbers or biomass, which can be inverted).

Priority 3: Sampling and population estimates

Random sampling with quadrats: use random coordinates, count organisms, calculate mean per quadrat, scale up using the formula: population estimate = mean per quadrat × (total area ÷ quadrat area).