The Three Main Digestive Enzyme Groups

Enzyme groupSubstrateProductsProduced byWorks in
Amylase (carbohydrase)StarchMaltose (then glucose)Salivary glands, pancreasMouth, small intestine
ProteaseProteinsAmino acidsStomach (pepsin), pancreas (trypsin)Stomach, small intestine
LipaseLipids (fats/oils)Glycerol + fatty acidsPancreasSmall intestine

Supplement: additional enzymes:

  • Maltase: produced by the wall of the small intestine. Breaks maltose → glucose.
  • Sucrase: breaks sucrose → glucose + fructose.
  • Lactase: breaks lactose → glucose + galactose.

The Role of Bile

Bile is NOT an enzyme: it is a green-yellow alkaline fluid produced by the liver, stored in the gall bladder, and released into the duodenum (first part of the small intestine).

Bile has two functions:

  1. Emulsification of fats: bile breaks large fat droplets into many smaller droplets, increasing the surface area for lipase to act on. This speeds up fat digestion.
  2. Neutralisation of stomach acid: bile is alkaline and neutralises the hydrochloric acid from the stomach, creating the slightly alkaline pH (7–8) needed for pancreatic enzymes to work.

Key point: Emulsification is a physical change, not a chemical one: bile does not chemically break down fat molecules.

Why Digestion Is Necessary

Large food molecules must be digested because:

  • They are too large to pass through the wall of the small intestine into the blood.
  • They are insoluble: they cannot dissolve in blood plasma for transport.
  • Digestion breaks them into small, soluble molecules (glucose, amino acids, fatty acids, glycerol) that can be absorbed and transported.

Types of digestion:

  • Mechanical digestion: physical breakdown (teeth chewing, stomach churning) increases surface area for enzymes.
  • Chemical digestion: enzymes hydrolyse bonds in food molecules, breaking them into smaller molecules.